Butyl Sepharose High Performance is an aliphatic hydrophobic interaction chromatography (HIC) medium, designed for intermediate and polishing step purification steps when high resolution has priority

• Aliphatic HIC resin designed for intermediate purification and polishing steps
• Selectivity similar to Butyl Sepharose 4 Fast Flow but higher resolution due to the smaller 34 µm bead size
• Relatively high ligand density for high dynamic binding capacity even at relatively low salt concentrations
• BioProcess resin supported for industrial applications
• The hydrophilic nature of the base matrix ensures low levels of non-specific binding.

Butyl Sepharose High Performance is a member of the Cytiva range of HIC resins for intermediate purification and polishing of proteins. Butyl Sepharose High Performance is based on highly crosslinked, 34-µm agarose beads modified with aliphatic butyl groups via uncharged, chemically stable ether linkages. It is a truly hydrophobic resin displaying a minimum of ionic interactions. Butyl Sepharose High Performance resins is particularly well suited for intermediate purification and polishing steps providing high resolution due to the small particle size.