Butyl Sepharose 4 Fast Flow is a well established, standard aliphatic hydrophobic interaction chromatography (HIC) resin for capture and intermediate purification of larger proteins.

• Standard aliphatic HIC resins based on Sepharose Fast Flow base matrix derivatized via uncharged, chemically-stable ether linkages.
• Optimized for the separation of larger proteins in capture and intermediate purification steps
• BioProcess resin supported for industrial applications and well-established in approved processes
• The hydrophilic nature of the base matrix ensures low levels of non-specific binding.

Butyl Sepharose 4 Fast Flow is part of the Sepharose Fast Flow HIC platform, which has been an industrial standard for HIC chromatography during recent decades. Butyl Sepharose 4 Fast Flow is based on cross-linked 4% agarose. The aliphatic ligand is immobilized to the base matrix with an ether linkage. The ligand contains no charged groups, making true hydrophobic interaction chromatography possible, without interfering ionic effects.